000			04736nam a22001937a 4500
999			_c2288 _d2288
003			OSt
005			20190629101016.0
008			190629b ||||| |||| 00| 0 eng d
020			_a978-1-4939-3601-4
028			_bAllied Informatics, Jaipur _c6283 _d26/06/2019 _q2019-20
040			_aBSDU _bEnglish _cBSDU
082			_a612.3 _bIVA
100			_aIvanov, Andrei I (Editor)
245			_aGastrointestinal Physiology and Diseases: Methods and protocols
500			_aThis volume provides a comprehensive collection of classical and cutting edge protocols and techniques to examine the normal development and physiological functions of the gastrointestinal system and to model the most common digestive diseases. The chapters focus on diverse research topics including ex vivo systems to study gastrointestinal development and functions, in vivo imaging of the gastrointestinal tract, isolation and characterization of intestinal immune cells, and animal models of gastrointestinal inflammation and cancer. The Gastrointestinal Physiology and Diseases: Methods and Protocols book targets wide audience of physiologists, cell and developmental biologists, immunologists, and physician-scientists working in the field of gastroenterology and beyond. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Highly practical and clearly written, Gastrointestinal Physiology and Diseases: Methods and Protocols will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining normal functions and diseases of the gastrointestinal tract.
504			_aContents: Part I In Vitro and Ex Vivo Systems to Study Gastrointestinal Functions and Diseases 1. CRISPR/Cas9-Mediated Genome Editing of Mouse Small Intestinal Organoids 2. Lentivirus-Based Stable Gene Delivery into Intestinal Organoids 3. Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cells 4. An Air–Liquid Interface Culture System for 3D Organoid Culture of Diverse Primary Gastrointestinal Tissues 5. Organotypical Tissue Cultures from Fetal and Neonatal Murine Colon 6. Using Chamber Technique to Measure Intestinal Epithelial Permeability 7. HPLC-Based Metabolomic Analysis of Normal and Inflamed Gut 8. NMR-Based Metabolomic Analysis of Normal and Inflamed Gut 9. Analysis of microRNA Levels in Intestinal Epithelial Cells Part II Imaging Analysis of the Gastrointestinal System in Vivo 10. Detecting Reactive Oxygen Species Generation and Stem Cell Proliferation in the Drosophila Intestine 11. Imaging Inflammatory Hypoxia in the Murine Gut 12. Label-Free Imaging of Eosinophilic Esophagitis Mouse Models Using Optical Coherence Tomography 13. Near-Infrared Fluorescence Endoscopy to Detect Dysplastic Lesions in the Mouse Colon 14. Visualization of Signaling Molecules During Neutrophil Recruitment in Transgenic Mice Expressing FRET Biosensors 15. In Vivo Myeloperoxidase Imaging and Flow Cytometry Analysis of Intestinal Myeloid Cells Part III Isolation and Characterization of Intestinal Immune Cells 16. Macrophage Isolation from the Mouse Small and Large Intestine 17. Isolation and Functional Analysis of Lamina Propria Dendritic Cells from the Mouse Small Intestine 18. Purification and Adoptive Transfer of Group 3 Gut Innate Lymphoid Cells 19. Immunotherapy with iTreg and nTreg Cells in a Murine Model of Inflammatory Bowel Disease 20. Isolation of Eosinophils from the Lamina Propria of the Murine Small Intestine Part IV Animal Models of Gastrointestinal Inflammation and Injury 21. Investigation of Host and Pathogen Contributions to Infectious Colitis Using the Citrobacter rodentium Mouse Model of Infection 22. Murine Trinitrobenzoic Acid-Induced Colitis as a Model of Crohn’s Disease 23. Oxazolone-Induced Colitis as a Model of Th2 Immune Responses in the Intestinal Mucosa 24. The Mongolian Gerbil: A Robust Model of Helicobacter pylori-Induced Gastric Inflammation and Cancer 25. A Rapid Screenable Assay for Compounds That Protect Against Intestinal Injury in Zebrafish Larva Part V Animal Models of Gastrointestinal Cancer 26. AOM/DSS Model of Colitis-Associated Cancer 27. Characterization of Colorectal Cancer Development in Apc min/+ Mice 28. Modeling Murine Gastric Metaplasia Through Tamoxifen-Induced Acute Parietal Cell Loss 29. The Hamster Buccal Pouch Model of Oral Carcinogenesis
650			_aMedical
942			_2ddc _cBK